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詳細介紹
| 品牌 | absin | 供貨周期 | 一周 |
|---|
| 概述 | |
|---|---|
| 描述 | Beta-actin (PS1TP5-binding protein 1), also known as ACTB, PS1TP5BP1. Entrez Protein NP_001092. It is one of six different actin proteins. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton.Actins are highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Actin is ubiquitously expressed in all eukaryotic cells. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. |
| 別名 | A26C1A; A26C1B; ACTB; ACTB_HUMAN; Actin beta; Actin cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; Actx; b actin; Beta cytoskeletal actin; Beta-actin; BRWS1; E430023M04Rik; MGC128179; PS1TP5 binding protein 1; PS1TP5BP1; |
| 克隆類別 | Monoclonal |
反應種屬  | Human; Mouse; Hamster; Rat; Monkey |
| 來源宿主 | Mouse |
| 應用 | ELISA 1/10000, WB 1/500 - 1/2000, ICC 1/200 - 1/1000, FCM 1/200 - 1/400 |
| 特異性 | beta-Actin antibody detects endogenous levels of total beta-Actin |
| 性能 | |
|---|---|
| 保存方法 | Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
| 濃度 | 1mg/ml |
| 分子量 | 43KD |
| 功能 | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| 純化方法 | Affinity-chromatography |
| 亞細胞定位 | Cytoplasm,Cytoskeleton,Note:Localized in cytoplasmic mRNP granules containing untranslated mRNAs. |
| 序列 | MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQS KRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT QIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDL AGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSY ELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLS GGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFMWISKQ EYDESGPSIVHRKCF |
實驗結果圖
Figure 1: Western blot analysis using beta-Actin mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate.
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